• Reconstruction des fibres blanches cérébrales à partir de la dissection et recalage dans l'IRM post-mortem : pour la comparaison à la tractographie cérébrale par IRM de diffusion / Ilyess Zemmoura ; sous la direction de Christophe Destrieux et de Gilles V ( PDF )

The knowledge of the morphology of white matter fiber tracts, which connect distant cerebral areas, is essential to better understand brain functions. Diffusion MR tractography indirectly reconstructs this anatomy using complex mathematical algorithms. After a review of the existing methods for tractography validation, we propose an original method based on 3D reconstruction of dissected tracts. Our method, FIBRASCAN, used iterative surface acquisitions during dissection. The tracts were segmented on each surface and then reconstructed by stacking these surfaces. A rigid support allowed registration between surfaces and then registration to MRI. We demonstrated the accuracy of each reconstructing step, and the feasibility of our method on several tracts. In the last part of this work, the structure of white matter fibers and the changes induced by preparation and dissection were investigated using electron microscopy. We showed that dissection preserves the structure of axons and can thus be considered as a validation tool for tractography.

• Dates
  Published on June 25, 2015, Created on June 25, 2015