Actualité

Apport de la lipidomique dans l'étude de l'efficacité digestive chez les poulets

  • Recherche,
  • Santé-Sciences-Technologie,
Date(s)

le 8 novembre 2018

Publié dans Poult Sci.

Projet de recherche collaborative dirigé par le Prof. Mignon-Grasteau (BOA, INRA, Université de Tours, Nouzilly, France) auquel le Dr. Lydie Nadal-Desbarats et le Prof. Patrick Emond ont participé.

Does lipidomic serum analysis support the assessment of digestive efficiency in chickens?

Abstract

The increasing cost of conventional feedstuffs used in poultry diets has bolstered interest in genetic selection for digestive efficiency (DE) to improve the adaptation of the birds to various alternative feedstuffs. However, DE measurement through AMEn is time-consuming and constraining. To simplify selection for DE, the potential of serum composition to predict AMEn was evaluated based on 40 birds from two broiler lines (D+ and D-) divergently selected on the fecal AMEn of a difficult-to-digest wheat-based diet. Differences in serum coloration were suspected between the two lines, and thus a spectrophotometric analysis was carried out, revealing a significant difference in absorption between 430 nm and 516 nm, corresponding to the signature of orange-red lipophilic pigments such as xanthophylls. To go further, the liposoluble fraction of the serum was explored for its lipidome by mass spectrometry. Discriminant analysis revealed that a pattern of 10 metabolites, including zeaxanthin/lutein, can explain 82% of the lipidomic differences between the two lines. Colorimetry combined with lipidomics studies confirmed the relationship between digestive efficiency and serum composition, which opens up new possibilities for using it as a quick and easy proxy of digestive efficiency.

Contact :
Prof. Patrick Emond :