Actualité

Analyse quantitative du métabolisme du tryptophane chez la souris par LC-MS

  • Recherche,
  • Santé-Sciences-Technologie,
Date(s)

le 11 février 2019

Publié dans Talanta

Projet de recherche collaborative dirigé par le Prof. Patrick Emond

Validation of a global quantitative analysis methodology of tryptophan metabolites in mice using LC-MS

Abstract

In this study, we validated a method for quantifying 20 tryptophan (Trp) catabolites by liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) in 4 different matrices (urine, serum, intestinal contents and liver). The detection limit for all metabolites ranged between 0.015 and 11.25 nmol/L and the dynamic range of the calibration curves were adjusted to allow quantification of metabolites at endogenous levels. Matrix effects were evaluated using isotope labeled internal standards. Reproducibility in the 4 matrices was characterized by CV = 6.2% with an accuracy of 6.6%. Our method has been applied to the determination and quantification of 20 metabolites concentrations in 5 different mouse compartments (plus cecal contents). Our results show that our approach allows for a global exploration of the Trp metabolism by quantifying a large number of Trp metabolites, at the individual level by multi-matrix approach.

Copyright © 2018 Elsevier B.V. All rights reserved.

Keywords

#Cecal and #intestinal #contents, #Kynurenine pathway, #Liver #matrix, #Tryptophan pathway, #Validation methodology.

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